Does the PromoCell 3D Tumorsphere Medium XF (C‑28070) maintain the stem cell character of cancer stem cells?
During quality control, PromoCell does not assess the presence or maintenance of specific stem cell markers in tumorspheres cultured in 3D Tumorsphere Medium XF (C‑28070).
Functional approach used instead
PromoCell applies a functional assay concept by culturing tumorspheres over serial passages in C‑28070.
Tumorsphere formation requires key biological properties such as:
Therefore, the ability to form and maintain tumorspheres over multiple passages functionally indicates the presence of CSCs/CICs, without relying on predefined marker expression.
Scientific advantage
3D tumorsphere culture enables researchers to:
Functional approach used instead
PromoCell applies a functional assay concept by culturing tumorspheres over serial passages in C‑28070.
Tumorsphere formation requires key biological properties such as:
- Anoikis resistance
- Self‑renewal capacity
Therefore, the ability to form and maintain tumorspheres over multiple passages functionally indicates the presence of CSCs/CICs, without relying on predefined marker expression.
Scientific advantage
3D tumorsphere culture enables researchers to:
- Study CSC biology in a functional context
- Avoid dependence on CSC marker panels, which can vary between tumor types and experimental systems
Products relevant to this topic
New
3D Tumorsphere Medium XF
Cat No
C-28070
Serum-free and xeno-free medium for the cultivation of cancer cell lines as 3D tumorspheres.
274.00 CHF
3D Tumorsphere Medium XF, phenol red-free
Cat No
C-28075
Serum-free and xeno-free medium for the cultivation of cancer cell lines as 3D tumorspheres.
299.00 CHF