Should trypsin be warmed prior to use?
In our standard protocol, we recommend using trypsin at room temperature (RT). Using trypsin at RT helps to reduce the risk of over‑trypsinization and thereby minimizes the chance of irreversible cellular damage.
However, for some specific cell types and their corresponding media, detachment at 37°C is recommended to ensure efficient cell release.
The exact temperature and detachment conditions depend on the cell type and are clearly specified in the respective Product Manuals, which should always be followed.
However, for some specific cell types and their corresponding media, detachment at 37°C is recommended to ensure efficient cell release.
The exact temperature and detachment conditions depend on the cell type and are clearly specified in the respective Product Manuals, which should always be followed.
