Transfer from PDX to in vitro assays Xenograft models are extensively used to provide information about the safety and efficacy in drug development, especially for targeted cancer therapies. Patient-derived xenograft (PDX) mice are the preferred mouse tumor models, but cell line-derived xenografts (CDXs) and nude rat xenografts are also used. Nevertheless,…
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PDX mouse: Pushing the possibilities with ex vivo cell culture
Innovating mouse research such as PDX mouse models is key to improving cancer therapeutics. In translating scientific findings from mice to men, multiple techniques of collecting biological data are necessary. This article will explain how….
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3D cancer model
De-skilling 3D tumor models Tissue-engineered 3D cancer models have grown in popularity with recent advances in cancer research. 3D models are more biomimetic than 2D cell monolayers cultured on tissue culture plastic. Examples are spheroids, tumorspheres, organoids, PDX and lately, tumoroids, which differ by origin (cell line or patient-derived), size, the complexity of cellular architecture and tumor environment….
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Applications for our cancer media toolbox
We have developed a toolbox of cancer cell media to establish primary cancer cell lines from biopsies, develop 3D tumor models or build screening assays. Below you find the different application areas to discover solutions for your workflow and choose the right medium or combination of media to leverage your cancer research….
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Primary Cancer Cells: A Culture System Opens the Door to Individualized Therapy
Self-destruction of the body: Cancer turns normal body cells into their malignant counterpart that wants to keep on replicating by any means. Understanding the individual disease offers the potential for more effective therapies with fewer side effects. The new PromoCell Primary Cancer Culture System allows researchers to generate high-fidelity in vitro models of primary tumors….
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Immuno-oncology
A new in vitro strategy Immuno-oncology is a unique combination of oncology and immunology. By activating or amplifying the body’s natural defenses, immuno-oncology aims to boost the immune system to fight cancer better. Targeted antibodies or immunomodulators are well-known immunotherapies; adoptive cell therapy using CAR-T or TCR cells is particularly important,…
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Advancements and applications of tumor organoids in cancer research
Organoid models of patient-specific tumors are transforming our understanding of cancer heterogeneity and its implications for personalized medicine.
In this interview with BioTechniques, our experts, Janina Moros, Alexander Trampe and Zinnia Noor, discuss the benefits of tumor organoids, how to establish and culture them and their applications across different areas of cancer biology….
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From biopsy to assay development
Struggling to get high quality biopsies? Isolating patient-derived cancer cells from different types of biopsies (bone marrow biopsy, core needle biopsy, endoscopic biopsy, etc.) is the golden standard for cancer research. However, obtaining regular surgical biopsies from patients is a challenging prospect. Additionally, the variability of the quality of tissue samples can be pretty high….
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Cancer stem cells in tumor biology
How do cancer stem cells develop? What is their role in tumor biology? What are current approaches to target cancer stem cells? And what are innovative solutions for working with them? Find all the answers in our blog post….
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CSC therapy – towards new opportunities
CSCs as therapeutic targets Cancer stem cells (CSCs) are a small subset of tumor cells that are able to evade anti-tumor defense mechanisms and suppress anti-tumor immune responses by releasing soluble immunosuppressive factors, such as anti-inflammatory cytokines. CSCs appear to have multiple strategies to escape immunological destruction, including the acquisition of genetic and non-genetic alterations that reduce immune recognition,…
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Extracellular vesicles in cancer therapy
EVs in tumor development Extracellular vesicles (EVs), including exosomes, play a crucial role in tumor biology. Alongside modulating the tumor microenvironment and contributing to the malignant cascade (angiogenesis, the host immune system response, and metastasis), EVs are involved in chemoresistance. Docetaxel in prostate cancer, for example, may transport mRNA encoding drug-resistant proteins or carry miRNA to recipient cells and trigger the expression of specific target genes….
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Top tips for incorporating organoids into your research: expert interview
Our expert, Dr. Elfie Rödel, shares her top tips for incorporating organoids into your research in an interview with RegMedNet….
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