In which passage do you test cell type‑specific markers?
Information on the passage number used for testing cell type‑specific markers can usually be found in the Certificate of Analysis (CoA).
- For current CoAs, this information is listed under Results of Analysis → Identity and Purity → Test Method
- For older cell lots, it may be listed under Phenotypic Characterization
- For blood cells, marker analysis is performed directly after thawing, as these cells are not expanded in culture prior to testing
- Macrophages are typically analyzed 2-3 days after thawing and plating
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