My Normal Human Cells stopped growing and died after a few weeks in culture. What could be the reason?
Normal Human Cells are primary cells and therefore have a finite lifespan in vitro.
After a certain number of population doublings, they will stop proliferating, enter senescence, and eventually die. This is a normal and expected behavior of primary cells.
In addition to their limited lifespan, other factors can accelerate growth arrest or induce cell death, including:
After a certain number of population doublings, they will stop proliferating, enter senescence, and eventually die. This is a normal and expected behavior of primary cells.
In addition to their limited lifespan, other factors can accelerate growth arrest or induce cell death, including:
- Incorrect medium preparation (missing or wrong supplements)
- Suboptimal trypsinization conditions
- Microbial contamination (e.g. mycoplasma)
- Excessive passaging combined with high seeding density
- Insufficient gas exchange (e.g. when using non-vented caps)
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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.