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Can I reduce the seeding density for PromoCell M1 macrophages?

The recommended seeding density of 100,000 cells/cm² for PromoCell M1 macrophages is required to achieve a confluent cell layer, as these cells do not proliferate after seeding.
 
Reducing the seeding density
Yes. If a fully confluent monolayer is not essential for your assay, the seeding density can be reduced by a factor of 3 to 5.

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