What is the difference between PromoCell HUVEC single donor (C‑12200) and HUVEC pooled (C‑12203)?

Both products are Human Umbilical Vein Endothelial Cells (HUVEC) and are comparable in terms of culture conditions, passage number after thawing, and growth performance. The key differences relate to donor origin and preparation.
 
HUVEC single donor (C‑12200, C-12206)

• Isolated from one individual umbilical cord
• Expanded in primary culture and cryopreserved at subconfluency
• After thawing, cells are at passage 1 (P1)
• Represent the biological characteristics of a single donor

HUVEC pooled (C‑12203, C-12208)

• Cells are simultaneously isolated from 2-4 different umbilical cords
• Initially cultured in separate tissue‑culture dishes
• Cells are pooled after trypsinization, provided their growth rates are comparable
• After thawing, cells are also at passage 1 (P1)

Growth characteristics

• Doubling times are comparable for both products, typically 14–24 hours per population doubling
• HUVEC pooled cultures may show a slightly more heterogeneous morphology, with somewhat more elongated cells, compared to single‑donor HUVEC

Recommended culture media

Both HUVEC single donor (C-12200) and pooled HUVEC (C-12203) can be cultured in Endothelial Cell Growth Medium (C‑22010), or Endothelial Cell Growth Medium 2 (C‑22011). HUVEC 2, single donor (C‑12206), and pooled HUVEC 2 (C‑12208) are isolated directly in Endothelial Cell Growth Medium 2 and are therefore best cultured in Endothelial Cell Growth Medium 2 (C‑22011).