Technical library

The exact formulation of PromoCell basal media is proprietary information and is not publicly disclosed.
If you require information on the concentration of a specific component for your experiments, please contact PromoCell Scientific Support, who can advise you on a case‑by‑case basis.
 

The qualitative and quantitative composition of PromoCell supplements is provided:

• on the PromoCell website, and
• in the data sheets of the respective specialized media, where applicable.

If no detailed composition is specified, the formulation of the supplement is proprietary and confidential.

For optimal cell performance, we generally recommend culturing cells without antibiotics whenever possible. However, if the use of antibiotics is deemed necessary, please use the following final concentrations:
Penicillin: 100 U/ml + Streptomycin: 100 µg/ml
or
Gentamicin: 50 µg/ml + Amphotericin B: 50 ng/ml
 
Important note
The addition of antibiotics may reduce the growth rate of cells and can mask low‑level contamination. Antibiotics should therefore be used with caution, but may be helpful in situations where maintaining contamination‑free cultures is particularly challenging.

Our kidney cells are characterized by their epithelial cell morphology and by cytokeratin expression using a pan-cytokeratin antibody.

The difference between PromoCell HREpC and HRCEpC lies in the anatomical origin of the cells:

• HREpC (C‑12665) comprise a heterogeneous population of renal epithelial cells isolated from both the renal cortex and the renal medulla.
• HRCEpC (C‑12660) are exclusively derived from the renal cortex.

As a result, HREpC represent a broader mix of renal epithelial subtypes, while HRCEpC are restricted to cortex‑derived epithelial cells.

For the M2 Macrophage Generation Medium, it is extremely important that the shelf life of 2 weeks (after addition of the cytokines) is not exceeded; the yield will quickly decrease thereafter. It is best to use the M2 medium as fresh as possible to avoid discrepancies between M1 and M2 yield.

Most PromoCell subcutaneous Human White Preadipocyte (HWP) lots achieve a differentiation ratio of > 80-90% when adipogenic differentiation is induced at passage 2 (P2), directly after thawing.
For optimal differentiation performance, PromoCell recommends:

• using cells that have undergone no more than 4-5 population doublings, and
• performing differentiation without exceeding one passage after thawing.

As preadipocytes age in culture, their differentiation capacity gradually declines, resulting in lower differentiation ratios.

No. We do not recommend shortening the centrifugation time.
Centrifugation at 350×g for 15 minutes has been validated and tested by PromoCell and is also used during quality control testing.
Reducing the centrifugation time or g‑force may result in significant cell loss, as intact macrophages may not sediment completely.

Recommendation
To ensure reliable recovery of macrophages for flow cytometric analysis, please follow the validated centrifugation conditions of 15 minutes at 350×g.

The cells sink down to the bottom of the culture vessel but don't really attach. They retain a roundish morphology and can be rinsed off with culture medium easily.

PromoCell Blood and Blood Progenitor Cells are cryopreserved directly after isolation (= P0). They haven't been in culture before freezing.