How long does it take before osteoblasts produce mineralized nodules?
For mineralization assays, human osteoblasts (HOB) are cultured in Osteoblast Mineralization Medium (C‑27020).
Mineralization can typically be detected after approximately 3 weeks
Detection methods include:
Mineralization can typically be detected after approximately 3 weeks
Detection methods include:
- Ca‑45 incorporation, or
- Histochemical staining such as von Kossa or Alizarin Red, which visualize calcium deposition
