Advancing respiratory research: Introducing our new Airway Epithelial Cell Growth Media

Our new Airway Epithelial Cell Growth Media are now available, expanding our airway portfolio with two enhanced formulations:

• Airway Epithelial Cell Growth Medium 2
• Airway Epithelial Cell Growth Medium XF (prf)

Our new media have been specifically designed to meet the evolving requirements of respiratory research. They provide researchers with defined, physiologically relevant, and regulatory-ready conditions to study complex airway biology. Our new BPE-free and xeno-free formulations allow researchers to obtain reliable data across a range of applications, from basic studies to translational research.

Both formulations provide distinct advantages to support airway epithelial research across 2D, 3D, and air-liquid interface (ALI) cultures:

Airway Epithelial Cell Growth Medium 2

• BPE-free formulation: Supports physiologically relevant airway epithelial morphology and function without the need for small molecules.
• Enhanced growth rate: Promotes increased population doublings across all three types of airway epithelial cells.
• Seamless ALI integration: Complements the ALI-Airway Medium for a complete BPE-free system.
• 3D culture ready: Proven to sustain high TEER values (≥ 500 Ω*cm² for ≥ 14 days) in combination with our BPE-free ALI-airway medium.

Airway Epithelial Cell Growth Medium XF (prf)

• Xeno-free and physiologically relevant: Ideal for regulatory-compliant applications.
• Batch-to-batch consistency: Minimizes variability and enhances reproducibility.
• Regulatory-ready: Provides comparable performance to BPE-containing medium and can be upgraded through our Custom GMP Services.
• Phenol red-free: Clean formulation without non-functional additives.

These advanced formulations offer researchers reproducible and physiologically relevant cell culture conditions. By doing so, they enable high-quality respiratory research, from basic studies to applications that meet regulatory standards.

Expert insight 

Dr. Lisa Hamelmann
Product Manager Cells & Media

“Air-liquid interface cultures are a cornerstone of respiratory research, but their success depends on stable and reproducible conditions,” explains Lisa. “With the introduction of BPE-free and xeno-free airway media, researchers gain access to defined systems that support epithelial growth, differentiation, and barrier formation — essential for advancing studies in respiratory biology and drug development.”