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M1-Macrophage Generation Medium XF
Serum-free and xeno-free medium for generation of M1 macrophages from fresh monocytes.
Our Macrophage Generation Media have been developed for the efficient generation of monocyte-derived macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. The M1-Macrophage Generation Medium XF contains GM-CSF and allows for the generation of M1-polarized Macrophages. Our M1-Macrophage Generation Medium XF is a ready-to-use medium including cytokines for the directed differentiation of M1-like polarized MDM.
The Macrophage Media XF are serum-free and xeno-free formulations for use with freshly isolated cells. Due to the utilization of exclusively synthetic, recombinant or plant-sourced materials, human serum albumin, purified from human plasma, is the only non-recombinant protein contained in this medium.
Although all our are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine macrophages. View a list of references where this medium was used with other species and cell types.
Components:
- Basal Medium M1 (250 ml)
- SupplementMix M1 (for 250 ml)
- CytokineMix M1: GM-CSF (for 250 ml)
After addition of the SupplementMix or SupplementPack to our basal medium do I have to add FCS you obtain the complete growth medium. No further supplementation with serum or growth factors is required. Please note: Our media do not contain antibiotics. If you wish to use antibiotics you can add penicillin/streptomycin or gentamicin/amphotericin B at standard concentrations. Addition of antibiotics can however reduce the doubling time of the cells up to 30-40%.
The qualitative and quantitative composition of the supplements can be found on our website and in the data sheets of the specialized media. When there is no such information specified; the composition of the supplements is confidential.
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Holotomography-Monitored Phagocytosis Assay of M1 MacrophagesApplication Notes Go To Resource
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Human Blood Cells TypesBrochures Go To Resource
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Macrophage Cell CultureApplication Notes Go To Resource
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Macrophage polarization: plasticity in health and diseasePosters Go To Resource
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Macrophages Subtypes: Polarization, Activation and PlasticityVideos Go To Resource
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Monocyte-derived macrophages: Customized in vitro large-scale differentiation from human PBMCApplication Notes Go To Resource
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Standardized culture of assay-ready and fully functional human primary macrophagesApplication Notes Go To Resource
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Differentiation of M1- or M2-Macrophages from PBMC/MonocytesApplication Notes Go To Resource
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