How can I test whether my cells are infected with mycoplasma?
Several established methods are available for the detection of mycoplasma contamination in cell cultures, including:
PCR‑based mycoplasma detection is widely used because it:
- Culture‑based methods: Growth on agar plates or in liquid/semi‑solid media
- Microscopy‑based methods: DNA staining (e.g. DAPI)
- Immunological methods: Detection using mycoplasma‑specific antibodies
- Biochemical assays
- PCR‑based assays
PCR‑based mycoplasma detection is widely used because it:
- Is highly sensitive
- Detects all mycoplasma species commonly found in cell culture
- Delivers results within 3-5 hours
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