Can I expand the human Mesenchymal Stem Cells prior to differentiating them?

Q: Can I expand the human Mesenchymal Stem Cells prior to differentiating them?

These cells are frozen at the end of 2 nd culture. Thawing and seeding results in passage 2 (3 rd culture). We recommend that they be used for differentiation experiments not later than passage 5. The differentiation potential of hMSC in vitro is reduced with ongoing population doublings, meaning the earlier differentiation is induced, the higher the differentiation rates.

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Accepted Answer

These cells are frozen at the end of 2^nd culture. Thawing and seeding results in passage 2 (3^rd culture). We recommend that they be used for differentiation experiments not later than passage 5. The differentiation potential of hMSC in vitro is reduced with ongoing population doublings, meaning the earlier differentiation is induced, the higher the differentiation rates.