Powered by BiozNormal Human Epidermal Melanocytes 2 (NHEM 2)
Primary human epidermal melanocytes isolated in PMA-free Melanocyte Growth Medium M2 from the epidermis of juvenile foreskin.
Primary Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of juvenile foreskin. Melanocytes are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10 % of the cells in the epidermis are melanocytes.
The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation. It is produced and stored in melanosomes, which are located close to the melanocyte membrane, but it can also be transferred to neighboring keratinocytes.
Our Normal Human Epidermal Melanocytes 2 are isolated using the serum-free and PMA-free Melanocyte Growth Medium M2.
Since PMA is a tumor promoting mitogen, it can interfere with experimental approaches. Therefore, we recommend using cells isolated in Melanocyte Growth Medium M2 and also using this medium for cultivation.
Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF) from the same donor are available on request.
- Our NHEM 2 are now also available from HLA-typed donors.
| Recommended plating density | 5000 - 10000 cells per cm2 |
| Passage after thawing | P2 |
| Tested markers | Mel-5 positive |
| Guaranteed population doubling | > 15 |
NOTE: We will be discontinuing our Normal Human Epidermal Melanocytes 2 (NHEM 2), effective October 2024.
However, we are pleased to offer our Normal Human Epidermal Melanocytes NHEM M3 as a high-quality alternative for your research needs.
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Melanocyte Growth Medium M2
Serum-free cell culture medium for the cultivation of juvenile and adult human melanocytes. Formulation is PMA-free.
Normal Human Epidermal Melanocytes M3 (NHEM M3)
Primary human epidermal melanocytes isolated in PMA-free Melanocyte Growth Medium M3. Available from juvenile and adult donors.