How do I best isolate RNA from PromoCell cell pellets (C-14**)?
There are two options for isolating RNA from cells stored in RNAlater Solution: 1) The solution is removed from the cells prior to extraction by centrifugation at 5,000 x g for 10 minutes at 4°C. Note: Because of the density of RNAlater® solution, greater centrifugal forces are required to spin down the cells. 2) If no pellet is visible after centrifugation, RNA can also be purified directly from the RNAlater® solution. This can be done by adding 2 ml of 10x lysis buffer, and proceeding normally.
Products relevant to this topic
NHEK.f Pellet
Cat No
C-14001
Cell pellet of NHEK from the epidermis of juvenile foreskin or adult skin in RNAlater® for subsequent RNA, DNA or protein analysis.
153.00£
NHEK adult Pellet
Cat No
C-14002
Cell pellet of NHEK from the epidermis of juvenile foreskin or adult skin in RNAlater® for subsequent RNA, DNA or protein analysis.
119.00£
NHEK.f pooled Pellet
Cat No
C-14003
Cell pellet of NHEK from the epidermis of juvenile foreskin or adult skin in RNAlater® for subsequent RNA, DNA or protein analysis.
136.00£
NHEK adult, pooled Pellet
Cat No
C-14004
Cell pellet of NHEK from the epidermis of juvenile foreskin or adult skin in RNAlater® for subsequent RNA, DNA or protein analysis.
102.00£
Human Follicle Dermal Papilla Cells (HFDPC), Cell Pellet in RNAlater
Cat No
C-14005
Cell pellet of HFDPC from human dermis of the scalp in RNAlater® for subsequent RNA, DNA or protein analysis.
290.00£
HUVEC Pellet, single donor
Cat No
C-14008
Cell pellet of HUVEC 2 isolated from the umbilical vein in RNAlater® for subsequent RNA, DNA or protein analysis.
109.00£
HUVEC Pellet, pooled donors
Cat No
C-14009
Cell pellet of HUVEC 2 isolated from the umbilical vein in RNAlater® for subsequent RNA, DNA or protein analysis.
107.00£
HUVEC Pellet
Cat No
C-14010
Cell pellet of HUVEC from the vein of the umbilical cord in RNAlater® for subsequent RNA, DNA or protein analysis.
109.00£