Do I need to use coated tissue‑culture vessels when culturing PromoCell Normal Human Cells?
For most PromoCell Normal Human Cells, coated culture vessels are not required. Cells are usually cultured successfully on standard tissue‑culture‑treated plastic.
However, coating is required for certain cell types and applications. Since extracellular matrix (ECM) coatings can influence cellular metabolism and gene expression, the same coating should be used consistently within an experimental series.
You should always consult the respective Instruction Manual to confirm whether coating is required and which matrix is recommended.
When is coating required?
However, coating is required for certain cell types and applications. Since extracellular matrix (ECM) coatings can influence cellular metabolism and gene expression, the same coating should be used consistently within an experimental series.
You should always consult the respective Instruction Manual to confirm whether coating is required and which matrix is recommended.
When is coating required?
- Mesenchymal Stem Cells (MSC) (C‑12974/C‑12971/C‑12977): Require fibronectin, vitronectin, or collagen type I when cultured in MSC Growth Medium XF (C‑28019) and/or during differentiation with PromoCell MSC Neurogenic (C-28015), Adipogenic (C-28016), or Osteogenic (C-28013) Differentiation Media.
- Human monocyte‑derived macrophages (C‑12914/C‑12916/C‑12915/C‑12917): Must be seeded on fibronectin‑ or vitronectin‑coated vessels when using M1/M2 Macrophage Generation Media XF.
- Osteoblast mineralization assays: Collagen type I‑coated plates are recommended when using Osteoblast Mineralization Medium (C‑27020).
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