Can I use PBS instead of HEPES BSS to wash cells before trypsinization?
For best results, we recommend using HEPES BSS to wash cells prior to trypsinization.
PromoCell Trypsin/EDTA (ready‑to‑use) and PromoCell TNS (Trypsin Neutralizing Solution) are both based on HEPES‑buffered Salt Solution (HEPES BSS). Using HEPES BSS for the washing step ensures optimal compatibility with these reagents.
Phosphate‑buffered saline (PBS) can also be used for washing; however, HEPES BSS is normally the preferred option.
PromoCell Trypsin/EDTA (ready‑to‑use) and PromoCell TNS (Trypsin Neutralizing Solution) are both based on HEPES‑buffered Salt Solution (HEPES BSS). Using HEPES BSS for the washing step ensures optimal compatibility with these reagents.
Phosphate‑buffered saline (PBS) can also be used for washing; however, HEPES BSS is normally the preferred option.
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