Technical library - RESOURCES

Our HCASMC (C-12221) are isolated from the large arteries, i.e. from

Right coronary artery
Left main coronary artery
Circumflex coronary artery and
Left anterior descending coronary artery.

Both, trypsin and accutase represent mixtures of different proteolytic enzymes. Trypsin is prepared from porcine pancreas, accutase from invertebrates. Accutase can replace trypsin for the detachment and dissociation of anchorage-dependent cells from surfaces and can also be used on suspension cells to reduce clumping in preparation for counting. The advantages of accutase over the traditional trypsin treatment are that it is more gentle and less damaging to cells (leading to increased viability) and does not contain any mammalian or bacterially derived proteins. Accutase is more thermolabile than trypsin and usually doesn't require an inactivation step.

We recommend to use the trypsin as well as the other detach solutions at room temperature to avoid overtrypsinization and irreversible cellular damage.

Upon arrival, the basal media should be stored between 4°C and 8°C, the supplements at -20°C.

Please do not freeze our cell culture media. Freezing can lead to irreversible precipitation of media components and the quality can no longer be assured.

Yes, you can use accutase to detach Normal Human Cells. Accutase acts very gently on the cells. Cell membranes and surface epitopes will not be harmed. It is therefore mostly used for applications that require unchanged surface markers, e.g. for flow cytometry, or for detachment of very sensitive cells.

The PromoCell trypsin 0.04% / EDTA 0.03% solution and the PromoCell TNS solution are both based on HepesBSS. Therefore, it is best to also use HepesBSS to wash the cells prior to trypsinization.

We recommend to use the DetachKit when subculturing our Normal Human Cells. It contains a HepesBSS washing buffer, trypsin 0.04% / EDTA 0.03% solution, and TNS, a trypsin inhibitor from soybean. Please note: Many of our culture media have low serum content or no serum at all. These media are not suitable to inactivate trypsin during subculture.

The population doubling time or generation time (t_g) is usually calculated during the logarithmic phase of growth. It specifies the time (t) in hours needed by the culture to double its cell number.

t_g = t / n

t = time (hrs) between 2 cell counts

n= number of population doublings

The Certificates of Analysis can easily be downloaded from our CoA search page. Simply type in the lot number indicated on the cryovial/TC-flask and click the SEARCH button.

You need to have experience working under sterile conditions and under a laminar flow hood. It is of advantage to have experience with other cell types and/or cell lines. If you are a beginner in cell culture and would like to establish a cell culture lab, we will assist you in working with PromoCell Normal Human Cells.

Serum turbidity is usually caused by cryoprecipitation of lipid components during freezing and thawing. The more times the serum is subjected to freeze/thaw cycles, the more turbidity is noticed.

It can be minimized by freezing the serum in aliquots at -20°C and thawing these aliquots individually at the time of use.