High-yield isolation of HUVECs in Endothelial Cell Growth Medium 2
Cat No
AP029
Here we show a procedure for a high-yield isolation of primary HUVECs in PromoCell’s Endothelial Cell Growth Medium 2 (ECGM2) and the comparison with a competitor medium.
Epi-Fluorescence mitochondrial imaging in live single cells and tumorspheres
Cat No
AP030
Mitochondria are the organelles that fuel life, which makes them extremely important for research. We offer a special dye for live-cell and fixed-cell imaging. Use this dye to boost your mitochondria research. Find out how.
Holotomography-monitored phagocytosis assay of M1 macrophages
Cat No
AP031
Phagocytosis is an essential process for pathogen clearance and is the subject of research for many immunologists. Find out more about PromoCell's phagocytosis assay for fluorescence microscopy and how you can implement this method in your lab.
Cell death and proliferation of pharmacologically relevant human primary cells with drug treatment
Cat No
AP032
Cytotoxic effects of a drug can be a crucial factor that differentiates a blockbuster from a failure. We assessed cytotoxic sensitivity of various primary cells in our portfolio using a high throughput-suitable assay.
Cancer stem cell selection from cancer cultures
Cat No
AP033
Traditional culture systems for cancer cells all share a lack of specific growth support for malignant cells. PromoCell's Primary Cancer Culture System is a reliable tool for depleting stromal cells, fibroblasts, and all other types of non-cancerous cells.
Unselective cell isolation from solid human tumor and xenograft biopsies
Cat No
AP034
Do your primary cells from solid human tumors and xenografts need broad growth support? Then find out how to employ the serum-free, non-selective Cancer Cell Line Medium XF.
Standardized culture of cancer cell lines under serum and xeno-free conditions
Cat No
AP035
Undefined media supplements have unwanted effects on cell cultures. Learn how to obtain more accurate and comparable results with PromoCell’s Cancer Cell Line Medium XF.
Isolation of patient-derived primary cancer cells
Cat No
AP036
Isolate tumor cells from patient samples and transform them into long-term cell cultures. Learn more about the primary cancer culture system and how it compares to other established culture methods.
Vascular cell culture under physiological oxygen tensions – implications for cellular redox homeostasis
Cat No
AP037
If the amount of reactive oxygen species exceeds the cells antioxidant capacity, your research subjects are stressed. Find out how you can shift your cell culture conditions and establish physiological conditions.
Isolation of fast proliferating and homogeneous keratinocyte populations
Cat No
AP038
Using the serum- and BPE-free Keratinocyte Growth Medium 3 for the isolation of keratinocytes showed an overall 50% increase of isolation efficiency with a significant smaller cell size and a homogenous morphology.
Isolation of patient-derived cancer stem cells from GIST liver metastasis
Cat No
AP039
Isolation of CSCs from tumor biopsies is critical for the development of new cancer therapies. Learn more about how our PCCS is supporting the isolation of cancer stem cells from liver metastases of gastrointestinal stromal tumors (GIST).
Cell cryopreservation in Cryo-SFM Plus
Cat No
AP040
This application describes a detailed protocol for freezing and thawing cells using our advanced defined, animal component-free and protein-free Cryo-SFM Plus cryopreservation medium.