Serum-free cell culture medium for differentiation of mesenchymal cells into neurogenic lineages.
Unit100 ml
Cat.No.
C-28015
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Currently In stock
We offer a complete Mesenchymal Stem Cell Media System including growth media, differentiation media and human mesenchymal stem cells (MSC).
We offers five MSC Differentiation Media to efficiently induce differentiation of MSC into adipogenic, chondrogenic (with and without inducers), osteogenic or neurogenic lineages, respectively.
Our Mesenchymal Stem Cell Neurogenic Differentiation Media is a serum-free medium developed for the directed differentiation of Human Mesenchymal Stem Cells (MSC) from bone marrow, the umbilical cord matrix (Wharton's Jelly) and adipose tissue into neurogenic lineages.
Figure 1.hMSC-BM after in vitro differentiation into neuronal cells using our Mesenchymal Stem Cell Neurogenic Differentiation Medium
Yes the specialized PromoCell media already contain the optimal amount of L-glutamine. Please don't add extra L-glutamine as this can be toxic for the cells.
A concentration > 10% FBS is needed to completely inactivate the trypsin. As most of PromoCell's growth media are serum-reduced or serum-free; the use of a trypsin inhibitor like TNS is highly recommended.
If antibiotics are deemed necessary the recommended final concentrations are:
100 U/ml penicillin + 100 µg/ml streptomycin or
50 µg/ml gentamicin + 50 ng/ml amphotericin B
Please note: Addition of antibiotics can reduce the growth rate of the cells.
After addition of the SupplementMix or SupplementPack to our basal medium do I have to add FCS you obtain the complete growth medium. No further supplementation with serum or growth factors is required. Please note: Our media do not contain antibiotics. If you wish to use antibiotics you can add penicillin/streptomycin or gentamicin/amphotericin B at standard concentrations. Addition of antibiotics can however reduce the doubling time of the cells up to 30-40%.
Yes you can aliquot the SupplementMix upon delivery and freeze down 2 or 4 individual aliquots at -20°C. This way you can prepare smaller volumes (2 x 250 ml or 4 x 125 ml) of complete culture medium and thus extend the time you can use the medium.
Upon arrival the basal media should be stored between 4°C and 8°C the supplements at -20°C.
Please do not freeze our cell culture media. Freezing can lead to irreversible precipitation of media components and the quality can no longer be assured.
The qualitative and quantitative composition of the supplements can be found on our website and in the data sheets of the specialized media. When there is no such information specified; the composition of the supplements is confidential.
The formulation of our basal media is proprietary information. If you need to know the concentration of a particular component for your experiments please contact the PromoCell Technical Customer Service.
It has been shown that phenol red has estrogenic properties. Phenol red-free media are therefore generally used in studies evaluating steroid hormone action in cultured estrogen-responsive cells (Berthois et al. 1986). Furthermore phenol red can also interfere with some analytical methods like photometric analyses.
The Growth Medium Kit allows customization of the end concentrations of growth supplements. It is therefore more flexible than the Medium "ready-to-use" and can eg. be used to prepare a starvation medium.
Please note: Modification of supplement concentrations may have an impact on cell growth. You should test in advance whether and for how long the cells can survive the altered culture conditions.
Adipogenic differentiation can be identified morphologically and without any staining by the formation of intracellular lipid vesicles.
In contrast; when MSC differentiate into bone cells; there is no significant change in morphology. It is recommended to perform Alkaline Phosphatase staining to detect osteoblastic differentiation or Alizarin Red S staining to show osteoblast mineralization.
Chondrogenic differentiation is generally performed as spheroids in 3-D cell culture and not in 2-D monolayer culture. Staining with Alcian Blue to visualize the differentiation process is indispensable.
Neurogenic differentiation can be detected using neuron specific markers (e.g. beta-3 tubulin; NeuN; MAP2) and by their typical neuronal morphology.
For differentiation protocols; please see attached Application Notes.
We have tested the differentiation capacity of our hMSC into adipocytes chondrocytes and osteoblasts over time and still see good differentiation rates after 10 population doublings i.e. at passage 5. However the differentiation potential declines with ongoing population doublings. To obtain optimal differentiation rates experiments should be performed as early in culture as possible.
PromoCell Cell Culture Media "ready-to-use" consist of basal medium and SupplementMix.
PromoCell Culture Media Kits consist of basal medium and SupplementPack.
Addition of the supplements (SupplementMix or SupplementPack; respectively) to the appropriate basal medium will result in identical growth media.
Metabolic Activity During Mesenchymal Stem cell (MSC) Differentiation
Mesenchymal stem cells: why optimizing manufacturing processes is key for a successful application
MSC reproducibility: Towards the standardization of Mesenchymal Stem Cells
Neurogenic differentiation and analysis of mesenchymal stem cells (MSC)
Cells in Action: Mitosis in Human Mesenchymal Stem Cells (MSCs)
Automated monitoring of metabolic activity and differentiation of human mesenchymal stem cells
Real-time analysis of stem cell proliferation during differentiation
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