Is it feasible to store the melanocytes for a second time in liquid nitrogen after subculture, similar to cell lines?

Upon arrival, you can either defrost the cells immediately and seed them in a TC vessel or store the vial in liquid nitrogen until needed.

It is possible to re-freeze normal human cells but PromoCell does not recommend it, because each freezing cycle leads to a loss in proliferation potential.

If you want to freeze them, please use our Cryo-SFM Plus (C-29920). It is also recommended to freeze the cells at an early passage.