Can I trypsinize the PromoCell Normal Human Cells at 37°C?

Our standard protocol recommends performing trypsinization at room temperature (RT).and to monitor cell detachment under the microscope. Prolonged trypsinization at 37°C can lead to irreversible damage of the cells.

However, in some cases, we recommend trypsinization at 37°C. The manual specifies the duration of trypsinization in these cases.