Can I thaw a vial of HBEpC and directly seed it on transwells for my ALI-experiment?
Yes, but remember to thaw and seed the cells in our growth factor containing Airway Epithelial Cell Growth Medium as the cells need to expand and proliferate for some days.
Always use a seeding density of 150.000 cells/cm2, even if you plate the cells on transwells directly after thawing. Do not forget to coat the inserts with 30 µg/ml Collagen Type I solution (e.g., Corning Inc®., product number 354236) before you seed the cells.