Do I need precoated flasks when culturing MSCs in PromoCell Mesenchymal Stem Cell Growth Medium XF (C‑28019)?
Yes. Pre‑coated culture vessels are required when culturing human mesenchymal stem cells (hMSCs) in PromoCell Mesenchymal Stem Cell Growth Medium XF (C‑28019).
Reason
MSC Growth Medium XF is a xeno‑free culture system that contains all necessary growth factors and supplements, but does not include attachment or spreading factors. Therefore, cells require an external coating to ensure proper adhesion.
Recommended coatings
Culture vessels must be pre‑coated according to the manufacturer’s instructions using one of the following options:
- Human fibronectin: 1 µg/cm²
- Human vitronectin: 0.5 µg/cm²
Alternatively, bovine fibronectin may also be used.
Reason
MSC Growth Medium XF is a xeno‑free culture system that contains all necessary growth factors and supplements, but does not include attachment or spreading factors. Therefore, cells require an external coating to ensure proper adhesion.
Recommended coatings
Culture vessels must be pre‑coated according to the manufacturer’s instructions using one of the following options:
- Human fibronectin: 1 µg/cm²
- Human vitronectin: 0.5 µg/cm²
Alternatively, bovine fibronectin may also be used.
Products relevant to this topic
Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC)
Cat No
C-12971
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)
Cat No
C-12974
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Adipose Tissue (hMSC-AT)
Cat No
C-12977
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Mesenchymal Stem Cell Growth Medium XF
Cat No
C-28019
Serum-free and xeno-free and cell culture medium for proliferation of mesenchymal stem cells. Fibronectin or vitronectin coating required.