Technical Library

Recent Entries in Technical Library

• Which plates does PromoCell recommend as 96-well U bottom suspension plates for chondrogenic differentiation of Human Mesenchymal Stem Cells?

  For chrondrogenic differentiation it is important that the cells do not adhere to the wells. During differentiation, the cells form spheroids which float in the medium. Therefore, there are no special requirements for the wells as long as they are U bottom shaped and suitable for suspension culture.

  Related Links and Documents

  • hMSC
  • Chondrogenic Differentiation and Analysis of MSC
• At what passage do PromoCell supply the cell pellets?

  The majority of PromoCell cell pellets (C-14**) are prepared 1 passage after thawing the cryopreserved cells.

  Examples:
  HUVEC and HUAEC correspond to P1 after thawing; therefore the pellets are frozen in P2.
  SMC or keratinocytes or epithelial cells correspond to P2 after thawing; therefore the pellets are frozen in P3.
  In contrast, our blood cells are cryopreserved directly after cell isolation. These pellets are prepared after thawing the cryovials with no further cultivation step.

• From what tissue do PromoCell isolate their HUtMEC (C-12295)?

  Our HUtMEC are isolated from the middle layer of the uterine wall (myometrium). The tissue donors were not pre-treated with hormones.

  Related Links and Documents

  • HUtMEC
• What is the composition of the Osteoblast Supplement (C-39615)?

  Our Osteoblast Supplement consists of FCS (10 % [v/v] final concentration) which is specifically tested to support optimal growth of Normal Human Osteoblasts.

  Related Links and Documents

  • Osteoblast SupplementMix
• What would be the likely impact of using PromoCell Fibroblast Growth Medium (C-23010) instead of DMEM + 10% FCS for the growth of juvenile fibroblasts (C-12300)?

  PromoCell Fibroblast Growth Medium is serum-free and therefore not subjected to the lot-to-lot variations observed with DMEM/10% FCS. Our Fibroblast Growth Medium therefore allows for much more standardized cell culture conditions.

  Related Links and Documents

  • Fibroblast Growth Medium
  • NHDF
• Can PromoCell supply melanocytes from asian donors?

  The majority of our skin tissue donors are caucasians. But occasionally we also get skin biopsies from asian and black donors.
  Please contact our Technical Customer Support if you need cells from a particular phototype or origin. They will check our inventory and send you a list of available cell lots.

  Related Links and Documents

  • Melanocyte cell culture
• How many bottles of medium will I need to grow 1 vial of HUVEC out to 15 population doublings?

  The amount of media needed per vial depends on the growth characteristics of the cells, the size of the TC vessels and the split ratios used, the frequency of media changes, the type of experiments you perform, etc. It is therefore difficult to give definite quantities. As a rough guideline, 1-2 bottles (500 ml each) are needed for 1 vial of HUVEC.

  Related Links and Documents

  • Endothelial Cell Growth Media
• Which cytokine concentrations does PromoCell recommend for activating M1/M2 macrophages?

  You may find all information regarding the activation and the cytokine concentrations in table 1 (page 5) of our Application Note.

  Related Links and Documents

  • Application Note
  • Macrophage Cell Culture
• What is the difference between PromoCell NHEK and NHEK GM3?

  The NHEK (primary human keratinocytes) are isolated in our serum-free Keratinocyte Growth Medium 2 (C-20011), the NHEK GM3 in our improved serum-free and BPE-free Keratinocyte Growth Medium 3 (C-20021). Both, NHEK and NHEK GM3 are available from single or from pooled donors isolated from the epidermis of juvenile foreskin or adult skin.

  Related Links and Documents

  • NHEK
  • NHEK GM3
• Can you drive organoid orientation (inward vs outward)?

  Yes. The orientation can be triggered by the use or lack thereof of ECM.

  Without the use of an ECM, the organoids will have a higher outward oriented ratio. You can find a protocol here https://pubmed.ncbi.nlm.nih.gov/30811997/, where cells were first embedded in ECM gel and afterwards ECM was dissociated, and free organoids were re-seeded in suspension without an ECM.

  * Please note that we have not tested this method in our labs and thus cannot guarantee it.

  Related Links and Documents

  • AppNote: Generation of Human Airway Organoids from Primary Cells