Technical Library

Recent Entries in Technical Library

• How should I handle PromoCell’s proliferating cells after arrival?

  Short description:
  Unpack the box and place the T25 flask(s) in the incubator for 3 hrs (closed cap). Then check confluency under the microscope.
  When the density is < 70%, aspirate the medium using sterile conditions and add 5-10 ml of the appropriate Growth Medium. The cells should be subcultured according to the subcultivation protocol given in the cells’ Instruction Manual once they have reached > 70 % confluency.

  Related Links and Documents

  • Subcultivation of primary cells
  • DetachKit
  • Accutase Solution
• I have a question related to PromoCell’s CD34+ Progenitor Cells (C-12921). Are they suspension cells or do they partially attach?

  It actually depends on the culture conditions whether the cells remain in suspension or attach to the surface.
  When grown in our serum-free, xeno-free HPC Expansion Medium XF (C-28021), the cells remain in suspension.

  Related Links and Documents

  • App. Note: Expansion of primitive Hematopoietic Progenitor Cells
  • Hematopoietic Stem Cell Culture
• Can rat or mouse SkMCs be cultured using the Skeletal Muscle Cell Growth Medium (C-23060)?

  Yes, the Skeletal Muscle Cell Growth Medium can also be used for rat, mouse and rabbit SkMC.
  We recommend to use the medium right after isolation. Cells that were isolated and cultured in a different medium beforehand may have adapted to the other medium. An abrupt medium change causes stress to the cells resulting in reduced growth rates and lower differentiation capacities.

  Related Links and Documents

  • Skeletal Muscle Cell Growth Medium
• Where can I find the composition of your basal media?

  The formulation of our basal media is proprietary information. If you need to know the concentration of a particular component for your experiments, please contact the PromoCell Technical Customer Service.

• From which organism originate the recombinant cytokines in Cytokine Mix E (C-39890)?

  All cytokines in Cytokine Mix E (human TPO, SCF, flt-3 ligand, and IL-3) are produced in E.coli. They are purified by chromatography, are free of endotoxins, and are tested for their biological activity.

  Related Links and Documents

  • Cytokine Mix E
• Are the PromoCell chondrocytes from orthopedic joint replacement surgery or other types of surgery?

  Our chondrocytes are derived from patients (~55-80 years) who underwent surgery for total endoprothesis of the hip or knee joint. In most cases this is necessary due to arthrosis. If the tissue shows macroscopic lesions, it is not used for cell isolation.

  Related Links and Documents

  • Human Chondrocytes
• Will RNAlater solution denature the proteins in the cell pellets?

  Yes, RNAlater Solution will denature proteins. Therefore, protein obtained from PromoCell cell pellets will be suitable for applications such as Western blotting or 2D gel electrophoresis, but not for applications that require native protein.

  Related Links and Documents

  • Cell pellets
• Should I store the cryopreserved cells in the liquid phase or gas phase of liquid nitrogen?

  In principle, both types of liquid nitrogen storage are acceptable, each having its advantages and disadvantages.

  • Liquid phase storage provides a consistent temperature of -196°C, a longer holding time and a greater vial capacity but involves the risk of contamination issues.
  • Storage in the gas phase is very safe with respect to contaminations but the holding time of the cells is shorter and the vial capacity is reduced.
• What is the approximate cell density of HFDPC (C-12071) at subconfluence?

  Typical cell densities are between 32,000 – 40,000 cells/cm² (approx. 800,000 – 1 million cells per T25-flask).

  Related Links and Documents

  • HFDPC
• Are the HPAEC harvested directly from the pulmonary artery or are these microvascular endothelial cells?

  Our HPAEC (C-12241) are harvested directly from the pulmonary artery. For their isolation, the vessel is explanted right after the position where the artery leaves the heart, including the bifurcation. HPAEC represent the innermost cell layer (i.e. the endothelial cells) of the pulmonary artery.
  We also supply pulmonary microvascular endothelial cells (HPMEC; C-12281) isolated from the capillaries of peripheral lung tissue.

  Related Links and Documents

  • HPAEC
  • HPMEC