Application Notes
Items 1-12 of 39 Results
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Air-Liquid Interface Culture of Nasal and Tracheal Airway Epithelial Cells
Generation of a 3D human airway model with primary human nasal and tracheal epithelial cells using our Air-Liquid Interface Medium (ALI-Airway).
Application Notes
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Expansion of primitive Hematopoietic Progenitor Cells
Researchers are facing difficulties during in vitro expansion of undifferentiated hematopoietic stem cells (HSC). Our Hematopoietic Progenitor Cell (HPC) Expansion Medium XF provides a highly optimized, and xeno-free culture system for human HSC.
Application Notes
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Automated monitoring of metabolic activity and differentiation of human mesenchymal stem cells
Detailed analysis of human stem cells offers the potential for the specific treatment of diseases by cell-based therapies. With an automated real-time analysis, we assess phenotypic changes during mesenchymal stem cell (MSC) differentiation.
Application Notes
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Xeno-free MSC culture: high yield, better viability and morphology
Are you trying to mimic in vivo conditions in your MSC cultures as closely as possible, but need to avoid serum? Then find out how the combination of PromoCell DXF medium and a unique coating called myMATRIX help you make your conditions highly physiological while maintaining reproducibility.
Application Notes
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MSC cultivation in single-use stirred tank bioreactors
MSCs have gained significant attention because of their therapeutic potential for many difficult-to-treat diseases. In this app note, we provide a protocol for (GMP) compliant large-scale production of MSCs in a single-use bioreactor using our MSC Growth Medium XF.
Application Notes
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Cancer organoid model
This application note describes a method for easy 3D organoid culture of Primary Human Dermal Fibroblasts (NHDF) and MCF-7 cells in our Cancer Cell Line Medium XF.
Application Notes
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Air-Liquid Interface Culture System for standardized respiratory research
Generation of a stable and functional 3D human airway model with primary human bronchial epithelial cells using the PromoCell Air-Liquid Interface Culture System.
Application Notes
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Isolation of Tumor Associated Macrophages (TAM) from Fresh Tumor Tissue
Tumor-associated macrophages (TAM) represent an important constituent of the tumor microenvironment (TME).
Application Notes
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Differentiation of M1- or M2-Macrophages from PBMC/Monocytes
Here we describe how the presence of PromoCell differentiation media encourages in vitro differentiation of mononcytes to macrophages. These then show either an M1-like or an M2-like polarization.
Application Notes
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Standardized culture of assay-ready and fully functional human primary macrophages
Cryopreserved human macrophages are now available as a reliable source of standardized cells, and our ready-to-use format provides full experimental flexibility. With this easy-to-handle application, your macrophage research will be more efficient.
Application Notes
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Monocyte-derived macrophages: Customized in vitro large-scale differentiation from human PBMC
Primary human macrophages are difficult to isolate and do not proliferate in culture. We offer a complete system for easy and cost-efficient generation of pure monocyte-derived macrophages.
Application Notes
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High-yield isolation of HUVECs in Endothelial Cell Growth Medium 2
Here we show a procedure for a high-yield isolation of primary HUVECs in PromoCell’s Endothelial Cell Growth Medium 2 (ECGM2) and the comparison with a competitor medium.
Application Notes