Is it possible to split differentiated adipocytes in order to perform more experiments?

Yes, differentiated adipocytes can generally be split.
However, it is important to understand the biological limitation of these cells:

• Loss of proliferative capacity after differentiation: Once adipocytes are fully differentiated, they no longer proliferate. As a result, the culture cannot be expanded further after splitting.
• Implications for experimental planning: Although trypsinization and passaging are technically possible, this step does not increase cell numbers and may introduce unnecessary stress to the cells. In addition, we have observed in internal tests that, after detachment, freezing/thawing, and re‑plating of differentiated adipocytes, the lipid vesicles are no longer visible.

For this reason, it is recommended to plate the cells directly into the required culture vessels (e.g. multiwell plates) before initiating differentiation, so that splitting after differentiation is not necessary.