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Is it possible to differentiate M1 macrophages from PBMC in 96-well plates?

At PromoCell, we have not tested macrophage differentiation from PBMC in 96-well plates, but we know from users that it is possible.

According to a customer the mononuclear cells differentiate very well in the 96-well format. A plating density of 1 million PBMCs (without prior determination of monocyte content) per well has been shown to be optimal. The working volume in a 96-well plate is usually 100 µl.

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Ute Liegibel

CID-100645

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