print this article

I have two questions regarding the PCR Mycoplasma Test Kit I/C. It is recommended to use a 1.5% agarose gel for the analysis. Would it also be possible to use a 2% or 3% pre-cast agarose gel? And do you recommend to use a DNA ladder?

  1. It is recommended to use a 1.5% agarose gel for the analysis of the amplified DNA. Theoretically, it is also possible to use a 2% or 3% pre-cast agarose gel as long as it is running for a sufficient distance to separate the bands at ∼270 bp (positive control and mycoplasma- positive samples) and 479 bp (internal control).
  2. A DNA ladder is not mandatory but is recommended to correctly identify all bands. We recommend using a 100 bp ladder, but a 250 bp ladder or similar, which allows you to identify the 270 and 479 bp bands is also suitable.

About the Team

Ute Liegibel

CID-100645

Choose your Region

Please choose your region for an optimized website experience. So we can provide you with the most useful information for your country.

  1. North America
    1. Canada
    2. United States
  2. Europe
    1. Albania
    2. Austria
    3. Belgium
    4. Bosnia-Herzogovina
    5. Bulgaria
    6. Croatia
    7. Czech Republic
    8. Denmark
    9. Finland
    10. France
    11. Germany
    12. Greece
    13. Hungary
    14. Ireland
    15. Italy
    16. Liechtenstein
    17. Luxembourg
    18. Macedonia
    19. Monaco
    20. Netherlands
    21. Norway
    22. Poland
    23. Portugal
    24. Romania
    25. Serbia
    26. Slovakia
    27. Slovenia
    28. Spain
    29. Sweden
    30. Switzerland
    31. United Kingdom
  3. Asia
    1. Bangladesh
    2. China
    3. Hong Kong (incl. Macau)
    4. India
    5. Indonesia
    6. Israel
    7. Japan
    8. Malaysia
    9. Nepal
    10. Singapore
    11. South Korea
    12. Sri Lanka
    13. Taiwan
    14. Thailand