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How much whole blood is required for 3 ml of the Lymphocyte Separation Medium in a 15 ml conical tube?

In general the 15 ml tubes are filled with 3 ml Lymphocyte Separation Medium 1077 and the 50 ml conical tubes with 15 ml Lymphocyte Separation Medium.

For the 15 ml tubes, it is recommended to use 3-8 ml, for the 50 ml tubes 15 – 30 ml of diluted* whole blood.

*1:2 -1:3 in PBS

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