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Following your protocol in a 96-well plate, how thick is the resulting Alginate Hydrogel matrix usually? And what are the maximum dimensions of an alginate hydrogel which still allow a sufficient exchange of nutrients and oxygen?

50 µl of Alginate Hydrogel 3D Cell Culture Matrix per well (96-well plate format) is what is recommended. We have not measured to see what the exact thickness of this is. But this is enough to provide sufficient exchange of nutrients and oxygen. Thicker matrices may not be advisable but you may try.

 

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