Does PromoCell recommend growing osteoblasts on type I collagen?

PromoCell generally cultures human osteoblasts (HOB) on uncoated tissue culture‑treated plastic. However, osteoblasts can also be cultured on extracellular matrix–coated surfaces, such as type I collagen or fibronectin.
 
Important experimental consideration
Please note that the type of extracellular matrix (ECM) coating can influence:

• the expression of certain genes (e.g. integrins), and
• downstream cellular metabolism and function

Because of this, PromoCell recommends:

• Using the same coating matrix consistently across a complete set of experiments
• Avoiding changes in surface coating within a study to ensure comparability and reproducibility