Can I use Growth Medium instead of TNS to inactivate trypsin when splitting cells?
In general, no. To completely inactivate trypsin, a serum concentration of > 10% FBS is required.
Because most PromoCell Growth Media are serum‑reduced or serum‑free, they do not contain sufficient serum to reliably stop trypsin activity. Therefore, the use of a dedicated trypsin inhibitor, such as Trypsin Neutralizing Solution (TNS), is strongly recommended.
Products relevant to this topic
DetachKit, 3 x 30 ml
Cat No
C-41200
DetachKit for gentle and effective detachment of adherent primary human cells. Contains Trypsin/EDTA (ready-to-use).
Keratinocyte Growth Medium 2 (Ready-to-use)
Cat No
C-20011
Serum-free cell culture medium for keratinocytes from the epidermis.
Keratinocyte Growth Medium 3
Cat No
C-20021
Serum-free and BPE-free cell culture medium for the cultivation of human keratinocytes from the epidermis of juvenile foreskin or adult skin.
Mammary Epithelial Cell Growth Medium (Ready-to-use)
Cat No
C-21010
Serum-free cell culture medium for epithelial cells from the mammary gland.
New
Airway Epithelial Cell Growth Medium 2
Cat No
C-21040
Serum-free and BPE-free cell culture medium for epithelial cells isolated from large air passages.
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Airway Epithelial Cell Growth Medium XF (prf)
Cat No
C-21050
Xeno- and serum-free cell culture medium for epithelial cells isolated from large air passages.
Airway Epithelial Cell Growth Medium (Ready-to-use)
Cat No
C-21060
Ready-to-use serum-freecell culture medium for epithelial cells from large air passages.
Small Airway Epithelial Cell Growth Medium (Ready-to-use)
Cat No
C-21070
Serum-free cell culture medium for epithelial cells from the distal respiratory tract.