VEGF (Vascular endothelial growth factor) is the only growth factor that stimulates vascular permeability. It promotes endothelial proliferation and survival, angiogenesis, vasculognesis and inhibits apoptosis. VEGF mitogenic activity is specific for endothelial cell and that makes it distinct among other growth factors. VEGF is thought to be important in the pathophysiology of neuronal and other tumors, by functioning as a promter of angiogenesis for human gliomas. Human VEGF occurs in several molecular variants and the 165 form is the most common form in most tissues. Biological activities of VEGF are not species-specific and glycosylation is not required for biological activity.
Recombinant human VEGF-165 produced in plant contains 165 amino acids and a 16 a.a. histidine-based tag for a total length of 181 a.a., and has a predicted molecular mass of 21.3 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 26-28 kDa in SDS-PAGE.
|Molecular Mass||21.30 kDa|
|Specific Activity||10⁵ IU/mg|
|Biological Activity||The ED₅₀ is determined by a cell proliferation assay measuring the dose-dependent effects of serial dilutions of recombinant human VEGF-165 on Human Umbilical Vein Endothelial Cells (HUVECs) and is comparable to VEGF-165 from other commercial sources. The ED₅₀ for this effect was found is typically below 10 ng/ml corresponding to a specific activity of >10⁵ units/mg. The optimal concentration should be determined for specific applications and cell lines.|
|Formulation||Sterile filtered through a 0.20 µm filter. Lyophilized from PBS (pH 7.20).|
|Reconstitution||Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. For long term storage it is recommended to add a carrier protein (0.10% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A).|
|Purity||Greater than 95% as determined by SDS-PAGE analysis. Purified product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction.|
|Endotoxin Level||Ultra-low endotoxin level: Endotoxin level is less than 0.01 ng per µg of product (0.05 EU/µg) as measured by turbidimetric kinetic assay.|
|Stability & Storage||The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.10% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze-thaw cycles.|