Reduced Glutathione (GSH) Assay Kit II

Fast and simple fluorometric assay for detecting reduced GSH levels in mammalian cells, while oxidized GSSG is not detected.

Reduced Glutathione (GSH) Assay Kit II
  100 assays
$ 659.00

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Reduced Glutathione (GSH) Assay Kit II

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Our Reduced Glutathione Assay Kit II is based on a specific enzymatic cycling method in the presence of GSH and a fluorophore. The reduction of the fluorophore produces a stable fluorescent product, the fluorescence of which is directly proportional to the amount of GSH in the sample and can be followed kinetically (Ex/Em=535/587 nm). The assay is simple, reproducible and can specifically detect as low as 8 pmol/well of reduced form of Glutathione (GSH) in a 100 µl reaction.
Our kits are the most specific and sensitive quantitative assays in the market because GSSG does not interfere with the assay. The kits include 5-sulfosalicylic acid (SSA) in order to protect samples’ GSH endogenous content (SSA acts as a deproteinizing and antioxidant agent).