HDAC-3 Immunoprecipitation (IP) & Activity Assay Kit
Simple, fast & convenient fluorometric assay for immunoprecipitation of the HDAC3 complex from cell & nuclear extract(s) and measurement of HDAC3 activity of immunoprecipitated complex/purified enzyme.
PromoCell’s HDAC-3 Immunoprecipitation (IP) & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC-3 complex from cells & tissues and to measure HDAC-3 activity fluorometrically. HAD-3 is immunoprecipitated from cell or nuclear extract(s) using HDAC-3 specific antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex reacts with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.