In PromoCell’s Glutamate Dehydrogenase Inhibitor Screening Kit, Glutamate is deaminated by GDH producing NADH, which converts a non-fluorescent probe to a fluorescent product in the presence of an enzyme mix. The end product is detected fluorometrically at Ex/Em = 535/587 nm. The kit provides a rapid, simple, sensitive plate based test, which is also suitable for high-throughput screening of GDH inhibitors.
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