The PromoKine Glucose Uptake Assay Kit III is based on 2-NBDG, a fluorescent deoxyglucose analog that can be taken up by cells through glucose transporters. However, 2-NBDG cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by this fluorescent glucose analog is proportional to glucose uptake by the cells and can be used to measure glucose uptake in cultured cells in response to insulin, growth factors etc. using fluorescent microscopy and flow cytometry. To validate the assay, the kit includes the natural phenol phloretin that inhibits glucose uptake.
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