Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P) which however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is, therefore, directly proportional to 2-DG (or glucose) uptake by cells. In the PromoKine fluorometric Glucose Uptake Assay Kit II, the accumulated 2-DG6P is enzymatically oxidized and coupled to a probe, which generates fluorescence in the presence of NADPH. This easy to use kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.
PromoCell’s facility remains operational and we continue to support researchers worldwide. We are proud of making a contribution to the fight against SARS-CoV-2 with our products. We are here to help - contact us. Dismiss