Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P) which however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is, therefore, directly proportional to 2-DG (or glucose) uptake by cells. In the PromoKine colorimetric Glucose Uptake Assay Kit I, the 2-DG6P is oxidized to generate NADPH, which can be determined by an enzymatic recycling amplification reaction. This easy to use kit can detect glucose uptake as low as 10 pmol/well and can be used for a variety of cell types.
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