Glucose (C6H12O6) is the primary biological fuel source used to generate the universal energy molecule, ATP. Glucose level is a key diagnostic parameter for many metabolic disorders and its measurement is very important in both research and drug discovery processes.
The PromoKine Glucose Assay Kit I provides direct measurement of glucose in various biological samples. Glucose Enzyme Mix oxidizes glucose specifically, to generate a product which reacts with a dye to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color or fluorescence is directly proportional to the amount of glucose present. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose levels during fermentation and glucose feeding in protein expression processes. The kit detects 1-10,000 μM of glucose in various samples.
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