Glutathione Reductase catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
PromoCell’s Glutathione Reductase Activity Assay Kit has been designed for measuring glutathione reductase (GR) activity in biological samples. It is highly sensitive and well suited for high-throughput. The assay is based on the GR-catalysed reduction of GSSG to GSH, which reacts with 5,5′-Dithiobis(2-nitrobenzoic acid) (DTNB) to generate the yellow TNB2-. The kit can detect 0.1 – 40 mU/ml GR in various samples.