Catalase is a ubiquitous antioxidant enzyme that is present in nearly all living organisms. It functions to catalyze the decomposition of hydrogen peroxide (H2O2) formed during oxidative stress to water and oxygen.
The PromoKine Catalase Activity Assay Kit provides a simple, direct, HTS-ready and highly sensitive assay for measuring Catalase activity in various biological samples (detecting pico-units of catalase). In the assay, catalase first reacts with H2O2 to produce water and Oxygen. The unconverted H2O2 then reacts with OxiRed probe to generate a product, which can be measured at 570 nm (colorimetric method) or at Ex/Em=535/587nm (fluorometric method). Catalase activity is reversely proportional to the signal.