Ascorbic Acid (Vitamin C) is a water-soluble antioxidant found in living organisms. It plays an important role in many biological processes and is a potent anti-oxidant, anti-inflammatory, and anti-viral agent, also having an effect as immune stimulant. It is critical for a variety of functions related to tissue growth and wound healing, neurotransmitter formation, blood cholesterol levels, as well as free radical neutralization. Being present in a wide variety of foods and biological specimens, it is important to be able to monitor variations of the ascorbic acid content reliably and accurate.
The PromoKine Ascorbic Acid Assay Kit provides a rapid, simple and sensitive method of detecting ascorbic acid in various biological samples. In this assay, a proprietary catalyst oxidizes ascorbic acid to a product that interacts with the ascorbic acid probe, generating a colorimetric (at λ = 570 nm) as well as fluorometric (Ex/Em = 535/587 nm) signal. The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.