Alanine Aminotransferase (ALT/SGPT) Activity Assay Kit
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Fast, convenient and sensitive colorimetric/fluorometric assay for measuring ALT activity in various biological samples
The Alanine Aminotransferase Activity Assay Kit also provides a fast, convenient, reliable and highly sensitive method that is compatible to high throughput applications. In this assay, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate, forming pyruvate and glutamate. The pyruvate is detected in a reaction that converts a nearly colorless probe to both, a colored (λmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm) product. Absorbance and fluorescence directly correlate to ALT activity in the sample.