Akt Activity Detection Kit

Complete kit for screening of Akt activity by Western blotting.

Akt Activity Detection Kit
  40 assays

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Akt Activity Detection Kit

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The PromoCell Akt Activity Detection Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3α as substrate. Phosphorylation of the GSK-3α can be analyzed by Western blot analysis using the phospho-GSK-3α specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.