Activin A, human, recombinant (HEK)
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Recombinant Human Activin A (HEK cell-derived)
Activin A is a TGF-beta family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors and in post-menopausal woman have been implicated in colorectal and breast cancers, respectively. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-beta antagonist, Follistatin.
Human recombinant Activin A produced in HEK cells is a non-glycosylated disulfide-linked homodimer, having a total molecular weight of 25 kDa. It has been purified by proprietary chromatographic techniques.
Please note: Recombinant human Activin A is identical to recombinant mouse and rat Activin A as numerous literary sources demonstrating the complete identity of the mature Activin A sequence among these three species.
| Molecular Mass | 25 kDa |
|---|---|
| Source | HEK cells |
| species | Human |
| Specific Activity | see below |
| Biological Activity | The specific activity was determined by the dose-dependent inhibition of proliferation of the MPC-11 cell line (mouse plasmocytoma cell line) and is typically 0.50 ng/ml. |
| Formulation | Lyophilized from a sterile-filtered protein solution in 1XPBS with no additives. |
| Reconstitution | Please Note: Always centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.10 – 1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. |
| Purity | Greater than 95% (determined by SDS-PAGE analysis). |
| Endotoxin Level | < 0.10 ng per µg of rHu Activin A (1EU/µg). |
| Stability & Storage | The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu Activin A should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.10% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze / thaw cycles. |