PromoCell’s 6-Phosphogluconate Assay Kit can be used with a variety of sample types. In this assay, 6-Phosphogluconate is converted to Ribulose-5-Phosphate by 6-Phosphogluconate Dehydrogenase in the presence of NAD, to form NADH, which reduces a probe and generates strong absorbance at 450 nm. This kit is simple, sensitive and easy to use, and can detect 6-Phosphogluconate levels lower than 20 μM.
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