4-1BB sReceptor (CD137), human, recombinant
Recombinant Human 4-1BB soluble Receptor (CD137)
4-1BB soluble Receptor, a member of the TNF superfamily of receptors, is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB Receptor binds to 4-1BBL to provide a co-stimulatory signal for T lymphocytes. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. The human 4-1BB Receptor gene codes for a 255 amino acid type I transmembrane protein containing a 17 amino acid N-terminal signal sequence, a 169 amino acid extracellular domain, a 27 amino acid transmembrane domain and a 42 amino acid cytoplasmic domain. Recombinant human soluble 4-1BB Receptor is a 167 amino acid polypeptide (14.0 kDa), which contains the cysteine rich TNFR-like extracellular domain of 4-1BB Receptor.
| Molecular Mass | 14 kDa |
|---|---|
| Source | E. coli |
| species | Human |
| Specific Activity | see below |
| Biological Activity | Determined by its inhibitory effect of the 41BBL mediated stimulation of IL-8 production by human PBMC. About 90% of inibition was seen using a concentration of 1µg for both 41BBL and 41BBR. |
| Formulation | Lyophilized from a sterile-filtered solution with no additives. |
| Reconstitution | Please Note: Always centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.10 – 1 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use. |
| Purity | Greater than 98% (determined by SDS-PAGE and HPLC analysis). |
| Endotoxin Level | < 0.10 ng per µg (1EU/µg) of 41BB Receptor. |
| Stability & Storage | The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted 41BB Receptor should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles. |